PhD: Civil Engineering
Concentration: Environmental Engineering
Global Biogeochemistry minor
M.S. Environmental Engineering
B.S. Aerospace Engineering, Magna Cum Laude
Post-Doctoral Research (2009 to 2012): MSU Center for Biofilm Engineering
Investigating electron transport within cells, between cells, and to extracellular matrices in the ubiquitous sulfate reducing bacterium, Desulfovibrio vulgaris Hildenborough (DvH).
Operating CDC Biofilm reactors with DvH wild-type pure cultures and select gene deletion mutants to learn:
o which genes and extra-cellular features (pili and flagella) are important to electron transport and biofilm formation for DvH;
o how differing electron donor/acceptor sources and ratios impact biofilm formation and electron transport.
Building and operating microbial fuel cells, using DvH as a biological anode to study electron transport and the role of biofilm and extracellular features in electron transport.
Using Field Emission Scanning Electron Microscopy (FE-SEM) to visualize extracellular features in biofilms.
Operating batch growth experiments to determine how aqueous chromium(VI) affects cell growth rate, viability, and the role of pili or flagella in chromium(VI) reduction.
Operated microcosms and batch culture experiments; designed, constructed and operated chemostats and recirculating packed columns to study reductive dechlorination of chloroethenes in mixed anaerobic Dehalococcoides-containing cultures.
Used above systems combined with anaerobic culturing techniques, gas chromatography (GC), high pressure liquid chromatography (HPLC), ion chromatography (IC), and quantitative polymerase chain reactions (qPCR) to investigate issues such as: Haldane inhibition of chloroethenes on their own dechlorination; toxicity of high concentrations of trichloroethene (TCE) and cis-1,2-dichloroethene (cDCE) to the dechlorination process; community shifts over time in long-term chemostat operation; gene expression under various conditions; and the comparison of attached to suspended growth for anaerobic reductive dechlorination of TCE.
Modeled dechlorination activity as Monod-based kinetics with inhibition, growth, decay and toxicity parameters for both batch and flow-through systems to describe observed activity
Researched the use of alkynes as tools for probing mechanisms of reductive dechlorination of chloroethenes
Environmental Engineering E.I.T. (October 1999 to October 2001) EGR & Associates, Eugene, OR
Groundwater quality assessment reporting for various landfills, including risk-based analysis of laboratory and field data
Collected well samples and oversaw groundwater monitoring well installation
Environmental Engineering E.I.T. (February 1998 to October 1999) Environmental Engineering, Inc., Blacksburg, VA
Designed, maintained, operated, and implemented UST remediation strategies for dual-phase and soil vapor extraction systems
Oversaw monitoring well installation
Collected groundwater samples and performed slug tests, estimated hydraulic parameters, and based on data collected.
Researched feasibility of bioremediation strategies for specific UST sites.
Sigma Xi, American Chemical Society, American Association for the Advancement of Science, Engineers Without Borders, Passed E.I.T. exam 1994
Sabalowsky, AR and L Semprini. 2010. Trichloroethene and cis-1,2-dichloroethene concentration-dependent toxicity model simulates anaerobic dechlorination at high concentrations: I. batch-fed reactors. Biotechnology and Bioengineering, 107(3):529– 539.
Sabalowsky, AR and L Semprini. 2010. Trichloroethene and cis-1,2-dichloroethene concentration-dependent toxicity model simulates anaerobic dechlorination at high concentrations: II. Continuous flow and attached growth reactors. Biotechnology and Bioengineering, 107(3):540–549.
Sabalowsky, AR. 2010. Spotlight: Modeling solvent toxicity with concentration- dependent increased cell decay. Biotechnology and Bioengineering, 107(3):fmv
Behrens, S., MF Azizian, PJ McMurdie, A Sabalowsky, ME Dolan, L Semprini, and AM Spormann. 2008. Monitoring Abundance and Expression of Dehalococcoides sp. Chloroethene Reductive Dehalogenases in a PCE-Dechlorinating Continuous Flow Column. Applied and Environmental Microbiology, 74:5695-5703.
Azizian, MF, S Behrens, A Sabalowsky, ME Dolan, AM Spormann, and L Semprini. 2008. Continuous-flow column study of reductive dehalogenation of PCE upon bioaugmentation with the Evanite enrichment culture. Journal of Contaminant Hydrology, 100:11-21.